Micropatterned Neuronal Networks in Culture: Simplicity or Complexity?

Bruce Wheeler
Department of Bioengineering
University of Illinois

Networks of living neurons can be grown in culture on top of multiple electrode arrays that permit stimulation and recording. With lithographic techniques their geometry can be defined suggesting that it is feasible to design customized neuronal networks that mimic brain circuits or execute logical functions. In this talk I review progress toward creating such networks, highlighting their means of creation and basic properties, but emphasizing the complexities involved in controlling even these simplified networks. Unavoidable complexities that are discussed include the roles of neurons vs. glia, different cell types, and different maturation states. Further, practical analysis of the electrical input/output data requires anticipation of the form, time scale, and complexity of the interrelationships.

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